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Dr. A. Villaverder: “High-Throughput Cell Motility Studies on Surface-Bound Protein Nanoparticles with Diverse Structural and Compositional Characteristics”

Dr. A. Villaverder: “High-Throughput Cell Motility Studies on Surface-Bound Protein Nanoparticles with Diverse Structural and Compositional Characteristics”

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  ACS Biomater. Sci. Eng.2019 https://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.9b01085   Abstract Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to perform a high-throughput study of the motility of NIH-3T3 fibroblasts under different conditions including the gradient steepness, particle concentrations, and area widths of patterned FGF-IBs, using for the data analysis a methodology that includes “heat maps”. From this analysis, we observed that gradients of concentr
Dr. A.Villaverde: “Controlling self-assembling and tumor cell-targeting of protein-only nanoparticles through modular protein engineering “

Dr. A.Villaverde: “Controlling self-assembling and tumor cell-targeting of protein-only nanoparticles through modular protein engineering “

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SCIENCE CHINA Materials Published online 19 September 2019 | https://doi.org/10.1007/s40843-019-9582-9 http://engine.scichina.com/publisher/scp/journal/SCMs/doi/10.1007/s40843-019-9582-9?slug=fulltext   Abstract Modular protein engineering is suited to recruit complex and multiple functionalities in single-chain polypeptides. Although still unexplored in a systematic way, it is anticipated that the positioning of functional domains would impact and refine these activities, including the ability to organize as supramolecular entities and to generate multifunctional protein materials. To explore this concept, we have repositioned functional segments in the modular protein T22-GFP-H6 and characterized the resulting alternative fusions. In T22-GFP-H6, the combination of T22 and H6
Dr. Mario Caceres: “Evolutionary and functional impact of common polymorphic inversions in the human genome”

Dr. Mario Caceres: “Evolutionary and functional impact of common polymorphic inversions in the human genome”

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Nature Communications 10,  Article number: 4222 (2019)   https://www.nature.com/articles/s41467-019-12173-x.epdf?author_access_token=cVjT1p3din4tGJbNLDyel9RgN0jAjWel9jnR3ZoTv0P-2IqhjSjJ3h8OoQACdg3_2Zs57EgxdseDbCAlUgZO9jSYZMidnZsRJxkAkaIfu2LAxppiY3VN8K2eAt2G6O7iHH_N5qqpM4UMjovqEWHu9A%3D%3D Open Access Abstract Inversions are one type of structural variants linked to phenotypic differences and adaptationin multiple organisms. However, there is still very little information about polymorphicinversions in the human genome due to the difficulty of their detection. Here, we develop anew high-throughput genotyping method based on probe hybridization and amplification, andwe perform a complete study of 45 common human inversions of 0.1–415 kb. Most inversionspromoted by homologous reco
Dr. Isidre Gibert i Dr. Xavier Daura: “Sulfonamide-based diffusible signal factor analogs interfere with quorum sensing in Stenotrophomonas maltophilia and Burkholderia cepacia”

Dr. Isidre Gibert i Dr. Xavier Daura: “Sulfonamide-based diffusible signal factor analogs interfere with quorum sensing in Stenotrophomonas maltophilia and Burkholderia cepacia”

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https://www.future-science.com/doi/abs/10.4155/fmc-2019-0015 https://doi.org/10.4155/fmc-2019-0015   Aim:Stenotrophomonas maltophilia (Sm) and Burkholderia cepacia complex (BCC) are Gram-negative bacterial pathogens, which are typically multidrug resistant and excellent biofilm producers. These phenotypes are controlled by quorum sensing (QS) systems from the diffusible signal factor (DSF) family. We aim to interfere with this QS system as an alternative approach in combatting such difficult-to-treat infections. Materials & methods: A library of sulfonamide-based DSF bioisosteres was synthesized and tested against the major phenotypes regulated by QS. Results & conclusion: Several analogs display significant antibiofilm activity while the majority increase the action of the...
Dr. Salvador Ventura:”Dual Binding Mode of Metallacarborane Produces a Robust Shield on Proteins”

Dr. Salvador Ventura:”Dual Binding Mode of Metallacarborane Produces a Robust Shield on Proteins”

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https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201902796 https://doi.org/10.1002/chem.201902796 Abstract An inorganic sandwich molecule, Na[Co(C2B9H11)2], able to produce vesicles through self‐assembly and known to produce strong dihydrogen‐bond interactions with amine groups is capable of interacting with proteins. This dual non‐bonding ability of Na[Co(C2B9H11)2] is what makes this molecule unique: it can be firmly anchored to a protein surface and is capable of extending over it. To prove this, the widely available bovine serum albumin (BSA), which has many pendant amino groups in its structure, has been taken as the model protein. It has been found that around 100 molecules of Na[Co(C2B9H11)2] preserve the native structure of BSA, while endorsing it with a significantly inc
Dr. A. Villaverde: Collaborative membrane activity and receptor-dependent tumor cell targeting for precise nanoparticle delivery in CXCR4+ colorectal cancer

Dr. A. Villaverde: Collaborative membrane activity and receptor-dependent tumor cell targeting for precise nanoparticle delivery in CXCR4+ colorectal cancer

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https://doi.org/10.1016/j.actbio.2019.09.002                                                            Abstract By the appropriate selection of functional peptides and proper accommodation sites, we have generated a set of multifunctional proteins that combine selectivity for CXCR4+ cell binding and relevant endosomal escape capabilities linked to the viral peptide HA2. In particular, the construct T22-GFP-HA2-H6 forms nanoparticles that upon administration in mouse models of human, CXCR4+ colorectal cancer, accumulates in primary tumor at levels significantly higher than the parental T22-GFP-H6 HA2-lacking version. The in vivo application of a CXCR4 antagonist has confirmed the prevalence of the CXCR4+tumor tissue selectivity over unspecific cell penetration, upon system
Dr. A Villaverde: Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

Dr. A Villaverde: Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

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https://onlinelibrary.wiley.com/doi/full/10.1002/advs.201900849   Abstract Two structurally and functionally unrelated proteins, namely Omomyc and p31, are engineered as CD44‐targeted inclusion bodies produced in recombinant bacteria. In this unusual particulate form, both types of protein materials selectively penetrate and kill CD44+ tumor cells in culture, and upon local administration, promote destruction of tumoral tissue in orthotropic mouse models of human breast cancer. These findings support the concept of bacterial inclusion bodies as versatile protein materials suitable for application in chronic diseases that, like cancer, can benefit from a local slow release of therapeutic proteins.                
Dra. Aurora Ruiz-Herrera: Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during Spermatogenesis

Dra. Aurora Ruiz-Herrera: Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during Spermatogenesis

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ARTICLE| VOLUME 28, ISSUE 2, P352-367.E9, JULY 09, 2019 Covadonga Vara; Andreu Paytuví-Gallart ; Yasmina Cuartero ;Paul D. Waters; Marc A. Marti-Renom; Aurora Ruiz-Herrera Open Access DOI: https://doi.org/10.1016/j.celrep.2019.06.037                                                                                                                         https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30803-4 Summary Mammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining in situ chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunopr
Dr. A. Villaverde: A CXCR4-Targeted Nanocarrier Achieves Highly Selective Tumor Uptake In Diffuse Large B-Cell Lymphoma Mouse Models

Dr. A. Villaverde: A CXCR4-Targeted Nanocarrier Achieves Highly Selective Tumor Uptake In Diffuse Large B-Cell Lymphoma Mouse Models

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http://www.haematologica.org/content/early/2019/06/25/haematol.2018.211490.article-info Aïda Falgàs, Victor Pallarès, Ugutz Unzueta, María Virtudes Céspedes, Irene Arroyo-Solera, María José Moreno, Alberto Gallardo, María Antonia Mangues, Jorge Sierra, Antonio Villaverde, Esther Vázquez, Ramon Mangues, Isolda Casanova Haematologica June 2019 : haematol.2018.211490; Doi:10.3324/haematol.2018.211490 Abstract One-third of diffuse large B-cell lymphoma patients are refractory to initial treatment or relapse after rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone chemotherapy. In these patients, CXCR4 overexpression (CXCR4+) associates with lower overall and disease-free survival. Nanomedicine pursues active targeting to selectively deliver antitumor agents to cancer