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Micro-cromosomes: evidència genòmica de l’origen dels cromosomes dels vertebrats

Micro-cromosomes: evidència genòmica de l’origen dels cromosomes dels vertebrats

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Els micro-cromosomes són components essencials dels cromosomes dels vertebrats actuals, inclosos els mamífers, en què es van fusionar i reorganitzar per formar els cromosomes presents en les diferents espècies. Així ho ha demostrat un estudi internacional amb participació de la UAB que ha rastrejat el seu origen i destí en ocells, rèptils i mamífers i ha identificat estructures cromosòmiques presents en l’origen dels vertebrats fa, almenys, 500 milions d’anys. Els resultats de la recerca, publicada en la revista PNAS, suposen un gran avanç en l’estudi de l’origen i la funció de l’organització del genoma de les espècies. Els micro-cromosomes són un tipus de cromosomes de mida molt petita, típics del genoma d’ocells, alguns rèptils i peixos, però absents en els mamífers actuals. Cons
Nanobiotechnology: “Tolerability to non-endosomal, micron-scale cell penetration probed with magnetic particles”

Nanobiotechnology: “Tolerability to non-endosomal, micron-scale cell penetration probed with magnetic particles”

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Colloids and Surfaces B: Biointerfaces : Volume 208, December 2021, 112123 https://doi.org/10.1016/j.colsurfb.2021.112123 Abstract The capability of HeLa cells to internalize large spherical microparticles has been evaluated by using inorganic, magnetic microparticles of 1 and 2.8 µm of diameter. In both absence but especially under the action of a magnet, both types of particles were uptaken, in absence of cytotoxicity, by a significant percentage of cells, in a non-endosomal process clearly favored by the magnetic field. The engulfed particles efficiently drive inside the cells chemically associated proteins such as GFP and human alpha-galactosidase A, without any apparent loss of protein functionalities. While 1 µm particles are completely engulfed, at least a fr
Soledad Carinelli i Silio Lima de Moura, guanyadors dels Premis Extraordinaris de Doctorat de la UAB

Soledad Carinelli i Silio Lima de Moura, guanyadors dels Premis Extraordinaris de Doctorat de la UAB

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Els dos doctorands han fet la tesi amb Maria Isabel Pividori Gurgo, Catedràtica a la facultat de Ciències i investigadora al Institut de Biotecnologia i Biomedicina (IBB) de la UAB. A més, la tesi de De Moura ha estat codirigida per Maria Mercè Martí Ripoll, també investigadora al IBB. Degut a la pandèmia, la cerimònia de l’any passat i aquest any es celebraran les dues, conjuntament, el proper mes de novembre. Els dos guardonats han estat premiats per les seves tesis doctorals: Carinelli amb la seva tesi “Biomarkers detection of global infectious diseases based on magnetic particles”, defensada originalment el setembre de 2019, i Lima de Moura amb “From preconcentration to rapid detection of exosomes as biomarkers in clinical diagnosis”, presentada el juliol de 2020. La cer
Protein Folding and Conformational Diseases: “Cryptic amyloidogenic regions in intrinsically disordered proteins: Function and disease association”

Protein Folding and Conformational Diseases: “Cryptic amyloidogenic regions in intrinsically disordered proteins: Function and disease association”

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Computational and Structural Biotechnology Journal; Volume 19, 2021, Pages 4192-4206 https://doi.org/10.1016/j.csbj.2021.07.019 Abstract The amyloid conformation is considered a fundamental state of proteins and the propensity to populate it a generic property of polypeptides. Multiple proteome-wide analyses addressed the presence of amyloidogenic regions in proteins, nurturing our understanding of their nature and biological implications. However, these analyses focused on highly aggregation-prone and hydrophobic stretches that are only marginally found in intrinsically disordered regions (IDRs). Here, we explore the prevalence of cryptic amyloidogenic regions (CARs) of polar nature in IDRs. CARs are widespread in IDRs and associated with IDPs function, with partic
Molecular Biology: “Functional Characterization of the Cell Division Gene Cluster of the Wall-less Bacterium Mycoplasma genitalium”

Molecular Biology: “Functional Characterization of the Cell Division Gene Cluster of the Wall-less Bacterium Mycoplasma genitalium”

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Front. Microbiol., 13 September 2021 | https://doi.org/10.3389/fmicb.2021.695572 It is well-established that FtsZ drives peptidoglycan synthesis at the division site in walled bacteria. However, the function and conservation of FtsZ in wall-less prokaryotes such as mycoplasmas are less clear. In the genome-reduced bacterium Mycoplasma genitalium, the cell division gene cluster is limited to four genes: mraZ, mraW, MG_223, and ftsZ. In a previous study, we demonstrated that ftsZ was dispensable for growth of M. genitalium under laboratory culture conditions. Herein, we show that the entire cell division gene cluster of M. genitalium is non-essential for growth in vitro. Our analyses indicate that loss of the mraZ gene alone is more detrimental for growth of M. genitalium than de
Biosensing and Bioanalysis: “Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays”

Biosensing and Bioanalysis: “Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays”

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Sensors 2021, 21(18), 5992; https://doi.org/10.3390/s21185992 Abstract This work addresses a method that combines immunomagnetic separation (IMS) and paper-based nucleic acid immunochromatographic assay for the sensitive detection of Mycolicibacterium fortuitum (basonym Mycobacterium fortuitum) In particular, the preconcentration of the bacteria was achieved by using magnetic particles modified with an antibody specific towards mycobacteria. Following the IMS, the bacteria were lysed, and the genome was amplified by double-tagging PCR, using a set of primers specific for the 16S rRNA gene for Mycobacterium. During the amplification, the amplicons were labeled with biotin and digoxigenin tags. Moreover, a comparative study of paper-based immunochromatographic platf
Protein Folding and Conformational Diseases: “Cryptic amyloidogenic regions in intrinsically disordered proteins: Function and disease association”

Protein Folding and Conformational Diseases: “Cryptic amyloidogenic regions in intrinsically disordered proteins: Function and disease association”

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https://doi.org/10.1016/j.csbj.2021.07.019 Abstract The amyloid conformation is considered a fundamental state of proteins and the propensity to populate it a generic property of polypeptides. Multiple proteome-wide analyses addressed the presence of amyloidogenic regions in proteins, nurturing our understanding of their nature and biological implications. However, these analyses focused on highly aggregation-prone and hydrophobic stretches that are only marginally found in intrinsically disordered regions (IDRs). Here, we explore the prevalence of cryptic amyloidogenic regions (CARs) of polar nature in IDRs. CARs are widespread in IDRs and associated with IDPs function, with particular involvement in protein–protein interactions, but their presence is also connected to a risk o
Protein Folding and Conformational Diseases: “Prion-like proteins: from computational approaches to proteome-wide analysis”

Protein Folding and Conformational Diseases: “Prion-like proteins: from computational approaches to proteome-wide analysis”

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FEBS Open Bio 11 (2021) 2400–2417 https://doi.org/10.1002/2211-5463.13213 Abstract Prions are self-perpetuating proteins able to switch between a soluble state and an aggregated-and-transmissible conformation. These proteinaceous entities have been widely studied in yeast, where they are involved in hereditable phenotypic adaptations. The notion that such proteins could play functional roles and be positively selected by evolution has triggered the development of computational tools to identify prion-like proteins in different kingdoms of life. These algorithms have succeeded in screening multiple proteomes, allowing the identification of prion-like proteins in a diversity of unrelated organisms, evidencing that the prion phenomenon is well conserved among species. Interestin
Nanobiotechnology: “Biparatopic Protein Nanoparticles for the Precision Therapy of CXCR4+ Cancers”

Nanobiotechnology: “Biparatopic Protein Nanoparticles for the Precision Therapy of CXCR4+ Cancers”

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Cancers 2021, 13(12), 2929  https://doi.org/10.3390/cancers13122929 Abstract The accumulated molecular knowledge about human cancer enables the identification of multiple cell surface markers as highly specific therapeutic targets. A proper tumor targeting could significantly avoid drug exposure of healthy cells, minimizing side effects, but it is also expected to increase the therapeutic index. Specifically, colorectal cancer has a particularly poor prognosis in late stages, being drug targeting an appropriate strategy to substantially improve the therapeutic efficacy. In this study, we have explored the potential of the human albumin-derived peptide, EPI-X4, as a suitable ligand to target colorectal cancer via the cell surface protein CXCR4, a chemokine receptor overexpress
Identifiquen nous gens relacionats amb una vida reproductiva més llarga en les dones

Identifiquen nous gens relacionats amb una vida reproductiva més llarga en les dones

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L'equip de recerca que dirigeix Ignasi Roig a l'IBB de la UAB. D'esquerra a dreta: Maria López Panadès, Andros Maldonado Linares, Mònica Ferrer Roda, Cristina Madrid Sandín, Yan Huang i Ignasi Roig Navarro. Una recerca internacional codirigida per la UAB ha identificat prop de 300 variacions gèniques que influeixen en la vida reproductiva de les dones. En l'estudi també s'han manipulat amb èxit gens clau en ratolins associats a aquestes variants tot incrementant la seva vida reproductiva. Els resultats, publicats a Nature, augmenten substancialment el coneixement sobre el procés d'envelliment reproductiu i aporten maneres de millorar la predicció de les dones que podrien arribar a la menopausa abans que altres. L’edat en què les dones passen per la menopausa és fonamen