{"id":4562,"date":"2022-04-06T12:23:22","date_gmt":"2022-04-06T11:23:22","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=4562"},"modified":"2022-04-06T12:23:22","modified_gmt":"2022-04-06T11:23:22","slug":"yeast-molecular-biology-the-toxic-effects-of-yeast-ppz1-phosphatase-are-counteracted-by-subcellular-relocalization-mediated-by-its-regulatory-subunit-hal3","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2022\/04\/06\/yeast-molecular-biology-the-toxic-effects-of-yeast-ppz1-phosphatase-are-counteracted-by-subcellular-relocalization-mediated-by-its-regulatory-subunit-hal3\/","title":{"rendered":"Yeast Molecular Biology: &#8220;The toxic effects of yeast Ppz1 phosphatase are counteracted by subcellular relocalization mediated by its regulatory subunit Hal3&#8221;"},"content":{"rendered":"\n<p><a href=\"https:\/\/febs.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Albacar%2C+Marcel\">Marcel Albacar<\/a>,<a href=\"https:\/\/febs.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Vel%C3%A1zquez%2C+Diego\">Diego Vel\u00e1zquez<\/a>,<a href=\"https:\/\/febs.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Casamayor%2C+Antonio\">Antonio Casamayor<\/a>,<a href=\"https:\/\/febs.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Ari%C3%B1o%2C+Joaqu%C3%ADn\">Joaqu\u00edn Ari\u00f1o<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/doi.org\/10.1002\/1873-3468.14330\">https:\/\/doi.org\/10.1002\/1873-3468.14330<\/a><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/feb2.v596.6.cover_.jpg\" alt=\"\" class=\"wp-image-4563\" width=\"381\" height=\"501\" srcset=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/feb2.v596.6.cover_.jpg 761w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/feb2.v596.6.cover_-228x300.jpg 228w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/feb2.v596.6.cover_-198x260.jpg 198w\" sizes=\"(max-width: 381px) 100vw, 381px\" \/><\/figure><\/div>\n\n\n\n<h2 id=\"d80327032\">Abstract<\/h2>\n\n\n\n<p>Overexpression of&nbsp;<em>Saccharomyces\u2009cerevisiae<\/em>&nbsp;protein phosphatase Ppz1 strongly impairs cell growth. Ppz1 is negatively regulated by its subunit Hal3, and Hal3 overexpression fully counteracts the toxic effects derived from high levels of the phosphatase. We show that Ppz1 localizes at the plasma membrane, and that co-expression of Hal3 recruits Ppz1 to internal membranes (mostly vacuolar). This effect is not observed in a catalytically impaired mutant of Ppz1. Disruption of intracellular trafficking by deletion of the ESCRT-0 component&nbsp;<em>VPS27<\/em>&nbsp;abolishes both Hal3-mediated relocalization of Ppz1 and normalization of cell growth, without affecting Ppz1 protein levels. We propose that Hal3 counteracts the toxic effects caused by excess of Ppz1 not only by inhibiting its enzymatic activity but also by recruiting the phosphatase to internal structures.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Marcel Albacar,Diego Vel\u00e1zquez,Antonio Casamayor,Joaqu\u00edn Ari\u00f1o https:\/\/doi.org\/10.1002\/1873-3468.14330 Abstract Overexpression of&nbsp;Saccharomyces\u2009cerevisiae&nbsp;protein phosphatase Ppz1 strongly impairs cell growth. Ppz1 is negatively regulated by its subunit Hal3, and Hal3 overexpression fully counteracts the toxic effects derived from high levels of the phosphatase. We show that Ppz1 localizes at the plasma membrane, and that co-expression of Hal3 recruits Ppz1 to [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":4563,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4562"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=4562"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4562\/revisions"}],"predecessor-version":[{"id":4564,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4562\/revisions\/4564"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/4563"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=4562"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=4562"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=4562"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}