{"id":4383,"date":"2021-10-05T18:26:00","date_gmt":"2021-10-05T17:26:00","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=4383"},"modified":"2021-10-05T18:30:49","modified_gmt":"2021-10-05T17:30:49","slug":"nanobiotechnology-tolerability-to-non-endosomal-micron-scale-cell-penetration-probed-with-magnetic-particles","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2021\/10\/05\/nanobiotechnology-tolerability-to-non-endosomal-micron-scale-cell-penetration-probed-with-magnetic-particles\/","title":{"rendered":"Nanobiotechnology: \u201cTolerability to non-endosomal, micron-scale cell penetration probed with magnetic particles&#8221;"},"content":{"rendered":"\n<h2 class=\"has-small-font-size\" id=\"publication-title\"><a href=\"https:\/\/www.sciencedirect.com\/science\/journal\/09277765\">Colloids and Surfaces B: Biointerfaces<\/a> : <a href=\"https:\/\/www.sciencedirect.com\/science\/journal\/09277765\/208\/supp\/C\">Volume 208<\/a>, December 2021, 112123<\/h2>\n\n\n\n<h2 class=\"has-small-font-size\" id=\"publication-title\"><a href=\"https:\/\/doi.org\/10.1016\/j.colsurfb.2021.112123\">https:\/\/doi.org\/10.1016\/j.colsurfb.2021.112123<\/a><\/h2>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/cover_colloidsandsurface.jpg\" alt=\"\" class=\"wp-image-4384\" width=\"328\" height=\"437\"\/><\/figure><\/div>\n\n\n\n<p id=\"sp0040\">The capability of HeLa cells to internalize large spherical <a href=\"https:\/\/www.sciencedirect.com\/topics\/physics-and-astronomy\/microparticles\">microparticles<\/a> has been evaluated by using inorganic, magnetic microparticles of 1 and 2.8&nbsp;\u00b5m of diameter. In both absence but especially under the action of a magnet, both types of particles were uptaken, in absence of cytotoxicity, by a significant percentage of cells, in a non-endosomal process clearly favored by the magnetic field. The engulfed particles efficiently drive inside the cells chemically associated proteins such as <a href=\"https:\/\/www.sciencedirect.com\/topics\/chemistry\/green-fluorescent-protein\">GFP<\/a> and human alpha-galactosidase A, without any apparent loss of protein functionalities. While 1&nbsp;\u00b5m particles are completely engulfed, at least a fraction of 2.8&nbsp;\u00b5m particles remain embedded into the cell membrane, with only a fraction of their surface in cytoplasmic contact. The detected tolerance to endosomal-independent cell penetration of microscale objects is not then restricted to organic, soft materials (such as bacterial inclusion bodies) as previously described, but it is a more general phenomenon also applicable to <a href=\"https:\/\/www.sciencedirect.com\/topics\/chemistry\/inorganic-material\">inorganic materials<\/a>. In this scenario, the use of magnetic particles in combination with external magnetic fields can represent a significant improvement in the <a href=\"https:\/\/www.sciencedirect.com\/topics\/biochemistry-genetics-and-molecular-biology\/internalization\">internalization<\/a> efficiency of such agents optimized as drug carriers. This fact offers a wide potential in the design and engineering of novel particulate vehicles for therapeutic, diagnostic and theragnostic applications.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Colloids and Surfaces B: Biointerfaces : Volume 208, December 2021, 112123 https:\/\/doi.org\/10.1016\/j.colsurfb.2021.112123 Abstract The capability of HeLa cells to internalize large spherical microparticles has been evaluated by using inorganic, magnetic microparticles of 1 and 2.8&nbsp;\u00b5m of diameter. In both absence but especially under the action of a magnet, both types of particles were uptaken, in [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":4384,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4383"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=4383"}],"version-history":[{"count":2,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4383\/revisions"}],"predecessor-version":[{"id":4387,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4383\/revisions\/4387"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/4384"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=4383"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=4383"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=4383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}