{"id":3824,"date":"2020-12-20T21:32:25","date_gmt":"2020-12-20T20:32:25","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=3824"},"modified":"2020-12-20T21:36:47","modified_gmt":"2020-12-20T20:36:47","slug":"nanobiotechnology-in-vivo-bactericidal-efficacy-of-gwh1-antimicrobial-peptide-displayed-on-protein-nanoparticles-a-potential-alternative-to-antibiotics","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2020\/12\/20\/nanobiotechnology-in-vivo-bactericidal-efficacy-of-gwh1-antimicrobial-peptide-displayed-on-protein-nanoparticles-a-potential-alternative-to-antibiotics\/","title":{"rendered":"Nanobiotechnology: \u201cIn Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics”"},"content":{"rendered":"

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\"\"Pharmaceutics<\/em>\u00a02020<\/b>,\u00a012<\/em>(12), 1217;\u00a0https:\/\/doi.org\/10.3390\/pharmaceutics12121217<\/a><\/h4>\n

Abstract<\/h2>\n<\/div>\n
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Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to conventional antibiotics. In this work, the antimicrobial peptide, GWH1, was N-terminally fused to two different scaffold proteins, namely, GFP and IFN-\u03b3 for its bacterial production in the form of such recombinant protein complexes. Protein self-assembling as regular soluble protein nanoparticles was achieved in the case of GWH1-GFP, while oligomerization into bacterial inclusion bodies was reached in both constructions. Among all these types of therapeutic proteins, protein nanoparticles of GWH1-GFP showed the highest bactericidal effect in an in vitro assay against\u00a0Escherichia coli<\/span>, whereas non-oligomerized GWH1-GFP and GWH1-IFN-\u03b3 only displayed a moderate bactericidal activity. These results indicate that the biological activity of GWH1 is specifically enhanced in the form of regular multi-display configurations. Those in vitro observations were fully validated against a bacterial infection using a mouse mastitis model, in which the GWH1-GFP soluble nanoparticles were able to effectively reduce bacterial loads.\u00a0View Full-Text<\/a><\/div>\n
Keywords:\u00a0<\/em>mouse mastitis model<\/a>;\u00a0antimicrobial peptide<\/a>;\u00a0protein nanoparticle<\/a>;\u00a0inclusion body<\/a>;\u00a0recombinant protein<\/a>;\u00a0Escherichia coli<\/i><\/a>;\u00a0Staphylococcus aureus<\/i><\/a>;\u00a0therapeutic protein<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

  Pharmaceutics\u00a02020,\u00a012(12), 1217;\u00a0https:\/\/doi.org\/10.3390\/pharmaceutics12121217 Abstract Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to conventional antibiotics. In this work, the antimicrobial peptide, GWH1, was N-terminally fused to two different scaffold proteins, namely, GFP and IFN-\u03b3 for its bacterial production in the form of […]<\/p>\n","protected":false},"author":65,"featured_media":3826,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/3824"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=3824"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/3824\/revisions"}],"predecessor-version":[{"id":3827,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/3824\/revisions\/3827"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/3826"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=3824"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=3824"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=3824"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}